Karthus Runes explained
There's one set of runes for Karthus on patch 14.22 that is the clear favorite for LoL players and pros alike. The best Karthus rune paths on the position 
Jungle are 
Domination as the primary path and 
Precision as the secondary path with a pick rate of 73%. Besides picking runes on Karthus you should learn how to play Karthus and if you are interested in pro matches take a look on how a pro builds Karthus. If you want to discuss 
Karthus then you should visit the champion subreddit.
Karthus runes from primary path
Domination improves burst damage and assassination tools on Karthus. Most of high elo players – and our LoL pros in the the major regions – have agreed that 
Dark Harvest is the best keystone rune to run on Karthus. This keystone rune gives you additional damage throughout the match. Other primary runes on Karthus are 
Cheap Shot, 
Eyeball Collection and 
Treasure Hunter.
Cheap Shot makes you deal more damage to enemies with impaired movement, which has great synergy with the setup of Karthus. Eyeball Collection is next up. This grants the user additional damage with every killing they score. Treasure Hunter is the final rune of the Domination path. This rune gives Karthus additional gold for takedowns. The additional gold is nothing to scoff at and can mean the difference between winning or losing a battle. Karthus will gain an additional 70 gold for the first takedown. Karthus acquires Bounty Hunter Stacks, which Karthus may use to get more takedowns. This rune's power will grow tremendously, and Karthus will become wealthy like never been before. Each time a fresh stack of Bounty Hunter Stacks is consumed, Karthus will get an additional twenty gold.
Karthus runes from secondary path
When it comes to your secondary path, most Karthus players prefer to go with Precision. Precision improves attack and sustained damage on Karthus troughout the match. On the Precision path, the best runes for Karthus are 
Presence of Mind and 
Last Stand.
Presence of Mind helps Karthus with the mana problems by restoring missing mana, if a champion is killed. With Last Stand you deal more damage while you are at low health.
FAQ for Karthus Runes
Which is the best keystone rune on Karthus?
The best keystone rune on Karthus is Dark Harvest. This Karthus runes setup is then referred as Karthus Dark Harvest.
What runes does Karthus use?
Runes from the paths Domination and Precision are the most used runes on Karthus. For the exact pick rates of Karthus runes used, you have to study the rune distribution presented.